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light sheet ultramicroscope microscopy  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec light sheet ultramicroscope microscopy
    Light Sheet Ultramicroscope Microscopy, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/light+sheet+microscopy/light+sheet+microscope+ultramicroscope+ii/pm40615713-96-13-17
    Average 90 stars, based on 1 article reviews
    light sheet ultramicroscope microscopy - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Medullary Kappa-Opioid Receptor Neurons Inhibit Pain and Itch Through a Descending Circuit
    Article Snippet: Whole brains and brainstem-spinal cord samples were immunolabeled with rabbit antiRFP (Rockland #600-401-379) at 1:200 during a 7 day incubation at 37 °C. .. Secondary antibody AlexaFluor 647- highly cross-adsorbed donkey anti-rabbit (Thermo Fisher, Cat #A-31573) was used at 1:400. iDisco-processed samples were then imaged using light sheet microscopy (Miltenyi Biotec Ultramicroscope II) with the 1x and 4x objectives. ..

    Article Title: Loss of vascular endothelial notch signaling promotes spontaneous formation of tertiary lymphoid structures.
    Article Snippet: For the B220 and Prox1/LYVE1 costaining, we incubated with anti-Prox1 (R&D AF2727, 1:100) and Anti-LYVE1 (R&D BAF2125, 1:100) for 2 days, washed for 24 h, and incubated with anti-goat-Cy3 (1:500) and anti-B220-AF647 (1:100) for another 2 days at 37 °C. .. Imaging using light sheet microscopy; for CD31/B220, we used a LaVision BioTec Ultramicroscope (Imaging Center Essen), for B220/Prox1, Lyve1 a LaVision BioTec UltraMicroscope II at Hannover Medical School (different wavelength laser equipment). .. For 3D Image reconstruction and movie animation, Imaris Software 7.6.5 Version I (Bitplane/Oxford Instruments) was used.

    Imaging:

    Article Title: Loss of vascular endothelial notch signaling promotes spontaneous formation of tertiary lymphoid structures.
    Article Snippet: For the B220 and Prox1/LYVE1 costaining, we incubated with anti-Prox1 (R&D AF2727, 1:100) and Anti-LYVE1 (R&D BAF2125, 1:100) for 2 days, washed for 24 h, and incubated with anti-goat-Cy3 (1:500) and anti-B220-AF647 (1:100) for another 2 days at 37 °C. .. Imaging using light sheet microscopy; for CD31/B220, we used a LaVision BioTec Ultramicroscope (Imaging Center Essen), for B220/Prox1, Lyve1 a LaVision BioTec UltraMicroscope II at Hannover Medical School (different wavelength laser equipment). .. For 3D Image reconstruction and movie animation, Imaris Software 7.6.5 Version I (Bitplane/Oxford Instruments) was used.



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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    Miltenyi Biotec light sheet ultramicroscope microscopy
    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence <t>microscopy.</t> Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.
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    Image Search Results


    (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

    Journal: bioRxiv

    Article Title: Most early-born subplate neurons persist as Layer 6b neurons in the adult mouse neocortex

    doi: 10.1101/2025.11.20.689634

    Figure Lengend Snippet: (A) Decrease rate of the neuronal (NeuN+) density in the SP during postnatal development. (The density in each stage – in P0)/(the density in P0) x100. (n = 3 brains, n = 18 images) (B) Immunohistochemical images of the coronal sections of Lapr1-GFP mouse brain at P0 (left) and 8 weeks (right) (C) Area of SP/L6b is defined by Lpar1-GFP expression (μm 2 ) (n=3). (D) Photographs of 4%PFA fixed mouse brain in P0 (top) and 8 weeks (bottom) (E) Anteroposterior (AP) length of the brain excluding the cerebellum (cm) (n=3) (F) Series of horizontal sections of 8-weel old Lpar1-GFP mouse brain, imaged by Light sheet fluorescence microscopy. Sections were taken at 200μm intervals. White arrows indicate the Lpar1-GFP+ SP layer.

    Article Snippet: Cleared whole brain tissues were imaged by Light sheet microscopy (Olympus, MVX10-LS), and the z-axis interval was 4 μm in the P0 sample and 9 μm in the 8 weeks.

    Techniques: Immunohistochemical staining, Expressing, Fluorescence, Microscopy